Mouse Anti-GD2 Recombinant Antibody (clone 3F8)
CAT#: PABL-101
Recombinant Mouse Antibody (3F8) is capable of binding to GD2 ganglioside, expressed in Chinese Hamster Ovary cells (CHO). It is a murine IgG3 with a moderate affinity for GD2 (KD = 5 nM).
Tested Data
SDS-PAGE
Figure 1 Mouse Anti-GD2 Recombinant Antibody (PABL-101) in SDS-PAGE
SDS-PAGE analysis of PABL-101 in non-reduced (Lane 1) and reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively. And non-reduced protein migrates as 180-245 kDa.
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Specifications
- Immunogen
- Human GD2
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human GD2
- Species Reactivity
- Human
- Clone
- 3F8
- Applications
- FC, ELISA, FuncS
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Cytotoxicity. For details, refer to published data.
Target
- Alternative Names
- disialoganglioside; GD2
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Excellent Quality Product
This antibody seems to be of excellent quality to me. The binding efficiency was excellent and it performed well in a variety of tests.
Sturdy Functioning
Throughout the months that I have been using this antibody, my results have always been reliable. Undoubtedly a trustworthy antibody.
Superb Technical Support
When I had queries regarding how to best optimize the settings for my test, the technical support was incredibly beneficial. Strongly advised.
Q&As
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How can I lessen the proteolytic degradation of my samples?
A: Add protease inhibitors to your lysis buffer to guard against proteolytic degradation. Samples should be kept on ice and treated as quickly as feasible to minimize degradation.
-
What part does validation of antibodies play?
A: Validation verifies that the antibody binds to the target antigen precisely and functions as intended in the particular circumstances of your application, ensuring consistent and trustworthy results.
-
Can I use primary antibodies again for my studies?
A: While it is theoretically possible, using multiple primary antibodies can decrease binding efficiency and raise background noise because of possible carryover. For optimal results, use fresh aliquots.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABL-101, RRID: AB_3111628)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone 3F8"
- CAT
- Product Name
See other products for "GD2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-770-sdAb | Recombinant Anti-Human GD2 VHH Single Domain Antibody | Neut, ChiP, IP, FUNC | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-731 | Anti-GD2 Recombinant Antibody (TAB-731) | Neut, ELISA, IF, IP, FuncS, FC, ICC | IgG1 - kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-L018R | anti-GD2 immunotoxin 14G2a (IgG)-RTA | Cytotoxicity assay, Functional assay |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-304CL | Anti-Human GD2 Recombinant Antibody (hu14.18K322A) | FC, FuncS | Antibody |
| PABX-059 | Human Anti-GD2 Recombinant Antibody (PABX-059) | FuncS | IgG1, κ |
| PABX-059-F (E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (PABX-059-F (E)) | FC, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-100 | Mouse Anti-GD2 Recombinant Antibody (clone 14G2a) | ELISA, WB, FuncS | Mouse IgG |
| PABZ-049 | Mouse Anti-GD2 Recombinant Antibody (clone ME36.1) | WB, FuncS | Mouse IgG |
| HPAB-1030WJ | Human Anti-GD2 Recombinant Antibody (HPAB-1030WJ) | ELISA | Chimeric (mouse/human) IgG |
| HPAB-1524WJ | Human Anti-GD2 Recombinant Antibody (clone KM8138) | ELISA, WB | Human IgG |
| HPAB-1525WJ | Human Anti-GD2 Recombinant Antibody (clone KM1138) | ELISA, WB | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-100 | Mouse Anti-GD2 Recombinant Antibody (clone 14G2a); Fab Fragment | ELISA, WB, FuncS | Mouse Fab |
| PFBL-101 | Mouse Anti-GD2 Recombinant Antibody (clone 3F8); Fab Fragment | FC, ELISA, FuncS | Mouse Fab |
| PFBZ-049 | Mouse Anti-GD2 Recombinant Antibody (clone ME36.1); Fab Fragment | WB, FuncS | Mouse Fab |
| HPAB-1524WJ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (clone KM8138) | ELISA, WB | Human Fab |
| HPAB-1525WJ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (clone KM1138) | ELISA, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBZ-049 | Mouse Anti-GD2 Recombinant Antibody (clone ME36.1); scFv Fragment | WB, FuncS | Mouse scFv |
| HPAB-0701-CN-S(P) | Human Anti-GD2 Recombinant Antibody (clone AB527); scFv Fragment | ELISA, FC, IHC, IF | Human scFv |
| HPAB-1030WJ-S(P) | Mouse Anti-GD2 Recombinant Antibody; scFv Fragment (HPAB-1030WJ-S(P)) | ELISA | Mouse scFv |
| HPAB-1524WJ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (clone KM8138) | ELISA, WB | Human scFv |
| HPAB-1525WJ-S(P) | Human Anti-GD2 Recombinant Antibody; scFv Fragment (clone KM1138) | ELISA, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-075CQ | Human Anti-GD2 Recombinant Antibody (TAB-075CQ) | ELISA, FC | Human IgM, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-074CQ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (TAB-074CQ-F(E)) | ELISA, FC | Human Fab |
| TAB-075CQ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (TAB-075CQ-F(E)) | ELISA, FC | Human Fab |
| TAB-076CQ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (TAB-076CQ-F(E)) | ELISA, FC | Human Fab |
| TAB-077CQ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (TAB-077CQ-F(E)) | ELISA, FC | Human Fab |
| TAB-078CQ-F(E) | Human Anti-GD2 Recombinant Antibody; Fab Fragment (TAB-078CQ-F(E)) | ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-029CN | Mouse Anti-GD2 Recombinant Antibody (clone 5F11) | WB | Mouse IgG |
| NS-030CN | Mouse Anti-GD2 Recombinant Antibody (clone 3G6) | WB | Mouse IgG |
| NS-029CN-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 5F11); Fab Fragment | WB | Mouse Fab |
| NS-030CN-F(E) | Mouse Anti-GD2 Recombinant Antibody (clone 3G6); Fab Fragment | WB | Mouse Fab |
| NS-029CN-S(P) | Mouse Anti-GD2 Recombinant Antibody (clone 5F11); scFv Fragment | WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-731 | Afuco™ Anti-GD2 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-731) | Neut, ELISA, IF, IP, FuncS, FC | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0125-FY63 | Human Anti-GD2 (clone 3F8) scFv-Fc Chimera | FC, ELISA, FuncS, ADCC, Cyt | Human IgG1, scFv-Fc |
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